课程概述

《动物微生物检验技术》是中国特色高水平畜牧兽医专业群的专业基础课程,共64学时,4学分。 落实立德树人根本任务,遵循职业教育技术技能人才成长规律,建立项目+任务+拓展”“岗课赛证融通课程结构模式。对接动物疫病防治等岗位群(岗),以智能化养殖场疫病防控综合性工作任务为整体,将教学内容整合为生产中的细菌病的实验室诊断(16学时)、病毒病的实验室诊断(16学时)、消毒与灭菌(8学时)、免疫防治(10学时)、免疫诊断(10学时)、生物制品及其应用(4学时)六个教学项目(课)。对接智能化养殖场岗位需求,引入动物疫病防控新方法、新技术、新规范,结合动物疫病检疫检验技能大赛等赛项标准(赛),参照兽医化验员等职业技能等级证书考核标准(证),融入思政、劳动和创新创业教育,将六个项目的教学内容重构为32个模块任务,分解岗位能力指标,细化模块化知识点151个、技能点44个。根据一带一路国家农牧企业人员培训等需求,增设大赛专项、课程思政、职教出海等3个拓展专项。挖掘课程思政元素,形成81个思政案例,有机融入课程内容,形成强农兴农、求真务实的课程思政主线。

"Animal Microbiological Examination Technology" is a basic professional course of high-level animal husbandry and veterinary professional group with Chinese characteristics, with a total of 64 hours and 4 credits. Implement the fundamental task of cultivating talents, follow the growth law of vocational education technical skills, and establish a "project + task + expansion" and "post course competition certificate" integrated course structure model. Docking post groups (posts) such as animal disease prevention and control, taking the comprehensive task of disease prevention and control in intelligent farms as a whole, The teaching content is integrated into six teaching projects (classes) : laboratory diagnosis of bacterial diseases in production (16 class hours), laboratory diagnosis of viral diseases (16 class hours), disinfection and sterilization (8 class hours), immune prevention and control (10 class hours), immune diagnosis (10 class hours), biological products and their application (4 class hours). To meet the job requirements of intelligent farms, introduce new methods, technologies and norms for animal disease prevention and control, combine the standards of animal disease quarantine and inspection skills competition (competition), refer to the assessment standards of vocational skill level certificates such as veterinary laboratory technicians, integrate ideological and political education, labor and innovation and entrepreneurship education, and restructure the teaching content of six projects into 32 module tasks. Decomposed post ability indicators, detailed 151 modular knowledge points, 44 skill points. According to the needs of personnel training of agricultural and animal husbandry enterprises in the "Belt and Road" countries, three expansion projects, including contest special, curriculum ideological and political, and vocational education going to sea, have been added. Excavating the ideological and political elements of the course, forming 81 ideological and political cases, organically integrating into the course content, forming the main line of "strengthening agriculture and rejuvenating agriculture, seeking truth and pragmatism".

预备知识

完成《动物解剖生理》《动物生物化学》等课程学习,了解基本解剖、生理、生物化学知识。

Complete courses such as Animal Anatomy and Physiology and Animal Biochemistry to understand basic anatomy, physiology and biochemistry.

授课大纲

动物微生物与免疫技术课程概述

课程概述

项目一 细菌病的实验室诊断

项目一任务导学单

1.1 课程概述

1.2 任务一 细菌形态和结构的认知

1.3 任务二 显微镜油镜的使用及细菌形态结构的观察

1.4 任务三 细菌涂片的革兰氏染色和病料触片的美蓝染色

1.5 任务四 细菌生理的认知

1.6 任务五 细菌的人工培养

1.7 任务六 细菌的分离培养及细菌的生化试验

1.8 任务七 细菌病的实验室诊断

实战1 鸡大肠杆菌病临床案例实验室诊断

实战2 副猪嗜血杆菌病临床案例实验室诊断

项目二 病毒病的实验室诊断

项目二任务导学单

2.1 任务一 病毒的认知

2.2 任务二 病毒形态和结构的认知

2.3 任务三 病毒增殖的认知

2.4 任务四 病毒的培养

2.5 任务五 病毒其他特性的认知

2.6 任务六 病毒的血凝试验

2.7 任务七 病毒的血凝抑制试验

2.8 任务八 病毒病的实验室诊断

实战1 新城疫临床案例实验室诊断

实战2 猪伪狂犬病临床案例实验室诊断

项目三 消毒与灭菌

项目三任务导学单

3.1 任务一 微生物在自然界分布的认知

3.2 任务二 外界环境因素对微生物影响的认知

3.3 任务三 微生物变异的认知

3.4 任务四 细菌的药物敏感性试验

项目四 免疫防治

项目四任务导学单

4.1 任务一 传染与免疫的认知

4.2 任务二 免疫系统的认知

4.3 任务三 抗原的认知

4.4 任务四 免疫应答的认知

4.5 任务五 体液免疫应答的认知

4.6 任务六 细胞免疫应答的认知

4.7 任务七 非特异性免疫应答的认知

项目五 免疫诊断

项目五任务导学单

5.1 任务一 血清学试验诊断

5.2 任务二 鸡白痢检疫

5.3 任务三 传染性法氏囊病卵黄抗体测定

5.4 任务四 变态反应诊断

项目六 生物制品及其应用

项目六任务导学单

6.1 任务一 生物制品的认知

6.2 任务二 生物制品的应用

专项一 大赛专项

赛项一 鸡新城疫抗体水平测定

赛项二 口蹄疫液相阻断酶联免疫吸附试验

赛项三 RT-PCR试验

专项二 课程思政专项

案例一 课程介绍思政案例

案例二 细菌病的实验室诊断课程思政案例

案例三 病毒病的实验室诊断课程思政案例

案例四 消毒与灭菌课程思政案例

案例五 免疫防治课程思政案例

案例六 免疫诊断课程思政案例

案例七 生物制品及其应用课程思政案例

专项三 职教出海培训专项—动物疫病防控新技术Vocational education to sea training special-New technology for animal disease prevention and control

任务三 培养基的制备及高压蒸汽灭菌器的使用Mission 3 Preparation of Medium and Use of Autodave Sterilizer

任务四 病料触片染色及细菌的分离培养Mission 4 Detection fo Bacteria in Diseased Materials

任务五 细菌的生化试验和药物敏感试验Mission 5 Biochemical and Drug Sensitivity Tests for Bacteria

任务六 病毒的鸡胚接种Mission 6 Chicken Embryos inoculation of Virus

任务七 鸡胚尿囊液的收获和病毒的血凝试验Mission 7 Harvest of Chicken Embryo Allantoic Fluid and HA Test for virus

任务八 血清学试验Mission 8 Serological Test

任务九 鸡的采血技术Mission 9 Chicken blood collection technology

任务十 新城疫抗体的测定Mission 10 Determination fo ND antibody

任务一 课程概述Mission 1 Overview of the course

任务二 细菌培养物涂片及革兰氏染色Mission 2 Gram's staining of the bacterial smear

参考资料

一、教材

1.授课教材

李舫,沈美艳.《动物微生物与免疫技术》.ISBN978-7-109-26099-3.北京:中国农业出版社(第三版),2023.03十四五职业教育国家规划教材)20198月在农业教育数字电子平台-智农书苑上线。

2.参考教材

1)陆承平.《兽医微生物学》.北京:农业出版社,第三版,2001.(普通高等教育十五国家级规划教材).

2)邓子新,陈峰.《微生物学》.北京:高等教育出版社,第一版,2017.

3)沈萍,陈向东.《微生物学》.北京:高等教育出版社,第八版,2016,《普通高等教育十二五国家规划教材》.

3.数字化教材

将传统的《动物微生物与免疫技术》纸质内容加入视频、动画、图片、PPT等资源的二维码,学生用手机扫描即可观看、学习。

一、Teaching Materials

1. Teaching materials

LI Fang,Shen Mei Yan.Animal Microbiology and Immunology.ISBN: 978-7-109-26099-3. Beijing: China Agriculture Press (3rd edition), 2023.03(the "14th Five-Year Plan" National Planning Textbook for Vocational Education), launched in August 2019 on the agricultural education digital electronic platform - Zhinong Library.

2. Refer to the textbook

(1) Lu Chengping. Veterinary Microbiology. Beijing: Agriculture Press, 3rd edition, 2001. (National Planning Textbook for the Tenth Five-Year Plan for General Higher Education).

(2) Deng Zixin, Chen Feng. Microbiology. Beijing: Higher Education Press, 1st edition, 2017.

(3) Shen Ping, Chen Xiangdong. Microbiology. Beijing: Higher Education Press, 8th Ed., 2016, "Twelfth Five-Year National Planning Textbook for General Higher Education".

3. Digital teaching materials

The traditional paper content of Animal Microbiology and Immune Technology is added to the two-dimensional code of videos, animations, pictures, PPT and other resources, and students can watch and learn by scanning it with their mobile phones.

二、信息化教学资源网站

1.国家级精品资源共享课《动物微生物》,爱课程网,网址:https://www.icourses.cn/sCourse/course_6142.html

2.畜牧兽医国家级专业教学资源库核心课程《动物微生物与免疫技术》,智慧职教,网址:https://www.icve.com.cn/portal/courseinfo?courseid=bbj5ak

3.动物检疫检验技术国家级专业教学资源库核心课程《动物微生物检验技术》,微知库,网址:https://xmzyk.36ve.com/?q=node/68139

二、Information teaching resources website

1.National high-quality goods resource sharing "animal microorganism, love course website, website: https://www.icourses.cn/sCourse/course_6142.html

2. Animal husbandry and veterinary state-level professional teaching repository core course animalmicrobes and the immune technology, vocational education wisdom, website:

https://www.icve.com.cn/portal/courseinfo?courseid=bbj5ak

3. Core course of National professional teaching resource bank of Animal Quarantine and Inspection Technology, "Animal Microbiological Inspection Technology",website:

https://xmzyk.36ve.com/?q=node/68139