Molecular Biology
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spContent=Genes are genetic material. Without heredity of genes, there would be no species. Without gene expression, there would be no growth of organisms. Without gene regulation, there would be no normal development of organisms. Would you like to know how genes are maintained, expressed and regulated? Would you like to understand the knowledge behind some useful technologies in biological research? Would you like to enhance your academic thinking and academic expression ability? Welcome to my course of Molecular Biology.
—— 课程团队
课程概述



Molecular Biology course explains the mechanisms of maintenance of the genome, expression of the genome and gene regulation at molecular level. The course also introduces the theoretical basis of some useful technologies applied in biological research.

 

Original Chinese version of this course has been online since 2015. Since then, it is highly praised by the learners. So far, there are more than 108,000 people have taken this course. In 2017, the course is awarded National Excellent Online Course on iCourse.

 

The major content of the course includes the structures of DNA, RNA and genome, DNA replication, RNA transcription, RNA splicing, protein translation, the genetic code, transcriptional regulation in prokaryotes and transcriptional regulation in eukaryotes. The content of this course are the basic knowledge required for biological research.

 

Currently, the teaching of this course is conducted as follows: first introduce the content in the courseware, then, go to the details of each point if necessary, and last, raise intriguing questions or ideas. If you have any advice on the teaching of this course, please let me know. I am willing to work with you and make this course better.


授课目标

1Help students master basic knowledge of molecular biology.

2Promote academic thinking.

3Enhance academic expression ability.

4Help students see how elaborate biological processes are.

5Stimulate interests in biology and biological research.


课程大纲
预备知识

Basic knowledge of biochemistry, genetics and cell biology.

参考资料

Textbook

Molecular Biology of the Gene, Sixth Edition

Cold Spring Harbor Laboratory Press

James Watson, Tania A. Baker, Stephen P. Bell, Alexander Gann, Michael Levine, Richard Losick

0-321-50781-9, 978-0-321-50781-5

 

References

Molecular Biology of the Gene, Seventh Edition

Cold Spring Harbor Laboratory Press

James Watson, Tania A. Baker, Stephen P. Bell, Alexander Gann, Michael Levine, Richard Losick 

0-321-85149-8, 978-0-321-85149-9

 

Molecular Biology, Fifth Edition

McGraw-Hill Education

Robert F Weaver

0-073-52532-4, 978-0-073-52532-7

 

Dintzis H.M. Assembly of the peptide chains of hemoglobin. Proc Natl Acad Sci USA. 47:247-261 (1961).

 

Dintzis H.M and Natughton M.A. Sequential biosynthesis of the peptide chains of hemoglobin. Proc Natl Acad Sci USA. 48:1822-30 (1962).

 

Dominique Ray-Gallet and Geneviève Almouzni. Mixing or not mixing? Science 328: 56-57, DOI: 10.1126/science.1188653 (2010).

 

John Bishop, John Leahy and Richard Schweet. Formation of the peptide chain of hemoglobin. Proc Natl Acad Sci USA. 46:1030-1038 (1960).

 

Julien Soudet, Pascale Jolivet, and Maria Teresa Teixeira. Elucidation of the DNA end-replication problem in Saccharomyces cerevisiae. Molecular Cell 53:954–964 (2014).


Meselson, M. S. & Stahl, F. W. The replication of DNA in Escherichia coli. Proc. Natl. Acad. Sci. 44:671-682 (1958).

 

Mo Xu, Chengzu Long, Xiuzhen Chen, Chang Huang, She Chen, Bing Zhu. Partitioning of Histone H3-H4 Tetramers during DNA replication-dependent chromatin assembly. Science 328:94-98, DOI: 10.1126/science.1178994 (2010).

 

Joachim Lingner, Julia Promisel Cooper, and Thomas R. Cech. Telomerase and DNA end replication: no longer a lagging strand problem? Science 269. 5230(1995): 1533-34.