细胞生物学

细胞生物学是生命科学重要的基础学科之一，是分子生物学与个体生物学之间承上启下的学科。本课程主要是让学生了解细胞的基本结构及其功能，熟悉细胞生命活动的基本特点及其分子机制，并了解当前细胞生物学的发展动态和国际前沿知识。本课程为中英文双语教学，即授课用中文，而PPT，教材及考试都为英文。适合对生物感兴趣，或者立志将来从事生命科学研究的学生进行学习。

本课程的学习过程包含：观看讲课视频、完成课后作业及讨论，参加期中，期末考试。

课程学习的最终成绩由一下几个部分组成：

(1) 课后作业成绩占20%

(2)期中考试成绩占40%

(3) 期末考试成绩占40%

在4月10日进行期中考试，5月22日进行期末考试，期中考试前的内容不再考察。

完成课程学习并考核合格(>=60分)的可获得合格证书，成绩优秀(>80分)的可获得优秀证书。

本课程的授课对象为大学二年级学生，前期课程应已修完生物化学、有机化学等基础学科。

1.Introduction of Molecular Cell Biology

1.1 Research objectives of the cell biology

1.2 Microscope and Cell theory

1.3 Major events in cell biology

2.Culturing,visualizing cells

2.1 Growing Cells in Culture
2.2 Monoclonal antibodies

2.3 Fluorescence Microscopy

2.4 Flow cytometry  

2.5 Spinning Disk Confocal Microscopy

2.6 FRAP and FRET

2.7 RNA interference

3.Biomembrane structure

3.1 The structrure of plasmic membrane

3.2 The lipid bilayer:Composition and structural organization

3.3 Forms of lipid molecules exist in water

3.4 Lipids Movement in biomembrane

3.5 Structure of Membrane Proteins

3.6 Synthesis of Phospholipids and Intracellular Movement

4.Transmembrane transport of ions  and small molecules

4.1 Overview of Transmembrane transport

4.2.Facilitated Transport of Glucose and Water

4.3 ATP –powered pumps

4.4  Na+/k+ pump

4.5 Ca2+ pump

4.6 K+ channel and the Resting membrane potencial

4.7 Cotransport by Symporters and Antiporters

4.8 Transcellular transport

5.Cellular Energetics

5.1 Overview of aerobic oxidation

5.2 Structure of Mitochondria

5.3 Mitochondrial Oxidation of Fatty Acids Generates ATP

5.4 The Electron Carriers and Electron Transport Chain

5.5 The Electron Transport and Generation of the Proton-Motive Force

5.6 Chemiosmotic theory  and Endosymbiont hypothesis  

5.7 Harnessing the Proton-Motive Force to synthesize ATP

6.Moving proteins into membrane and organells

6.1 Targeting Proteins to and Across the ER Membrane

6.2 Insertion of single-pass Proteins into the ER

6.3 Insertion of Multipass Proteins into the ER

6.4 Protein Glycosylation and Folding in the ER

6.5 Formation of disulfide bonds in the ER

6.6 Protein Quality Control in the ER

6.7Targeting of Proteins to Mitochondria

6.8 Targeting of Peroxisomal Proteins

7.Vesicular traffic,secretion and endocytosis

7.1 Molecular Mechanisms of Vesicle Budding and Fusion

7.2 Early Stages of the Secretory Pathway

7.3 Later Stages of the Secretory Pathway

7.4 Receptor-Mediated Endocytosis

7.5The structure and functions of Lysosomes

7.6 Directing Membrane Proteins and Cytosolic Materials to the Lysosome

8.Signal transduction

8.1 Brief Introduction of Cell Communication

8.2 Signaling Molecules and Receptors

8.3 G Proteins and G Protein-coupled Receptors (GPCR)

8.4 GPCR Mediated Signaling pathways

9.Signaling pathways that control  gene activity

9.1 Protein Tyrosine Kinases (RTKs) and RTK-Ras Signaling Pathway

9.2 Phosphoinositide Signaling Pathways

9.3 TGF-b Receptors and TGFb Signaling Pathways

9.4 Cytokine receptors and JAK-STAT Signaling Pathways

9.5 Signaling Pathways controlled by Ubiquitination

9.6 Integration of Multiple Signaling Pathways

10: I.Microfilaments: Actin Structures

10.1 Actin Filaments

10.2 Dynamic Assembly of Actin Filaments

10.3 Actin-Based Cellular Structures

10.4 Myosins and Myosin-Powered Movements

10.5 Mechanisms of Cell Migration

11. II Microfilaments: Microtubules and Intermediate Filament

11.1  Structure and Organization of Microtubule

11.2  Regulation of Microtubule Dynamics

11.3  Microtubule-Based Motor Proteins

11.4  Intermediate Filaments

11.5  Coordination of Cellular Cytoskeleton

12.Cell Nucleus

13.The Eukaryotic cell cycle

13.1. Overview of the cell cycle and its control

13.2. Model organisms and methods to study the cell cycle

13.3. CDK and Its Discovery

13.4. Regulation of CDK Activity

13.5. Commitment to the Cell Cycle and DNA Replication

13.6. Entry into Mitosis and Completion of Mitosi

13.7. Surveillance mechanisms in cell cycle regulation

13.8 Meiosis: A Special Type of Cell Division

14. Cell senescence

15.Integrating cells into tissues

15.1 An Overview on Cell-Cell and Cell-Matrix Adhesion

15.2 Type of Cell Junctions and Anchoring Junction

15.3 Tight junctions and Gap junctions

15.4 ECMs of The Basal Lamina

15.5 ECMs of Connective tissu

16.Stem cells, cell asymmetry and cell death

16.1 Early metazoan developmnt and embryonic stem cells

16.2 Stem Cells and Niches in Multicellular Organism

16.3 Mechanisms of cell polarity and asymmetric cell division

16.4 Cell death and its regulation

1.         Molecular Cell Biology, 7th Edition, Harvey Lodish/Arnold Berk, etc., 2013,  Macmillan                   Higher Education

2.           Molecular Biology of The Cell, 6th Edition, Alberts B., et al., John Wilson/Tim  Hunt, 2015,                Garland Science