厦门大学

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课程概述

细胞生物学是生命科学重要的基础学科之一,是分子生物学与个体生物学之间承上启下的学科。本课程主要是让学生了解细胞的基本结构及其功能,熟悉细胞生命活动的基本特点及其分子机制,并了解当前细胞生物学的发展动态和国际前沿知识。本课程为中英文双语教学,即授课用中文,而PPT,教材及考试都为英文。适合对生物感兴趣,或者立志将来从事生命科学研究的学生进行学习。


授课目标
我们期待通过本课程的双语教学,使同学们能熟练掌握细胞生物学的基本概念和基本原理。熟悉相关领域内的关键词汇,提高同学们的阅读英文文献的能力,使同学们获得运用所学知识去分析科技文献中数据,跟踪本学科领域内的最新进展的能力。
证书要求


本课程的学习过程包含:观看讲课视频、完成课后作业参加期中,期末考试。

课程学习的最终成绩由一下几个部分组成:

(1) 课后作业成绩占20%

(2)期中考试成绩占40%

(3) 期末考试成绩占40%

在第十三周进行期中考试,期中考试前的内容不再考察。

完成课程学习并考核合格(>=60)的可获得合格证书,成绩优秀(>80)的可获得优秀证书。

 




预备知识

本课程的授课对象为大学二年级学生,前期课程应已修完生物化学、有机化学等基础学科。


授课大纲

1.Introduction of Molecular Cell Biology

1.1 Research objectives of the cell biology

1.2 Microscope and Cell theory

1.3 Major events in cell biology

2.Culturing,visualizing cells

2.1 Growing Cells in Culture
2.2 Monoclonal antibodies

2.3 Fluorescence Microscopy

2.4 Flow cytometry  

3.Biomembrane structure

3.1 The structrure of plasmic membrane

3.2 The lipid bilayer:Composition and structural organization

3.3 Forms of lipid molecules exist in water

3.4 Lipids Movement in biomembrane

3.5 Structure of Membrane Proteins

4.Transmembrane transport of ions  and small molecules

4.1 Overview of Transmembrane transport

4.2.Facilitated Transport of Glucose and Water

4.3 ATP –powered pumps

4.4  Na+/k+ pump

4.5 Ca2+ pump

4.6 K+ channel and the Resting membrane potencial

4.7 Cotransport by Symporters and Antiporters

4.8 Transcellular transport

5.Moving proteins into membrane and organells

5.1 Targeting Proteins to and Across the ER Membrane

5.2 Insertion of single-pass Proteins into the ER

5.3 Insertion of Multipass Proteins into the ER

5.4 Protein Glycosylation and Folding in the ER

5.5 Formation of disulfide bonds in the ER

5.6 Protein Quality Control in the ER

5.7Targeting of Proteins to Mitochondria

5.8 Targeting of Peroxisomal Proteins

6.Vesicular traffic,secretion and endocytosis

6.1 Molecular Mechanisms of Vesicle Budding and Fusion

6.2 Early Stages of the Secretory Pathway

6.3 Later Stages of the Secretory Pathway

6.4 Receptor-Mediated Endocytosis

6.5 Directing Membrane Proteins and Cytosolic Materials to the Lysosome

7.Signal transduction

7.1 Brief Introduction of Cell Communication

7.2 Signaling Molecules and Receptors

7.3 G Proteins and G Protein-coupled Receptors (GPCR)

7.4 GPCR Mediated Signaling pathways

8.Signaling pathways that control  gene activity

8.1 Protein Tyrosine Kinases (RTKs) and RTK-Ras Signaling Pathway

8.2 Phosphoinositide Signaling Pathways

8.3 TGF-b Receptors and TGFb Signaling Pathways

8.4 Cytokine receptors and JAK-STAT Signaling Pathways

8.5 Signaling Pathways controlled by Ubiquitination

8.6 Integration of Multiple Signaling Pathways

                Midterm exam

9: I.Microfilaments: Actin Structures

9.1 Actin Filaments

9.2 Dynamic Assembly of Actin Filaments

9.3 Actin-Based Cellular Structures

9.4 Myosins and Myosin-Powered Movements

9.5 Mechanisms of Cell Migration

10. II Microfilaments: Microtubules and Intermediate Filament

10.1  Structure and Organization of Microtubule

10.2  Regulation of Microtubule Dynamics

10.3  Microtubule-Based Motor Proteins

10.4  Intermediate Filaments

10.5  Coordination of Cellular Cytoskeleton

11.The Eukaryotic cell cycle

11.1. Overview of the cell cycle and its control

11.2. Model organisms and methods to study the cell cycle

11.3. CDKs and Regulation of CDK activity

11.4. Commitment to the cell cycle and DNA replication

11.5. Entry into mitosis

11.6. Completion of mitosis: chromosome segregation and mitotic exit

11.7. Surveillance mechanisms in cell cycle regulation

12.Integrating cells into tissues

12.1 An Introduction on Integrating Cells into Tissues

12.2 An Overview on Cell-Cell and Cell-Matrix Adhesion

12.3 Type of Cell Junctions as presented in Epithelium

12.4Cadherins in adherens junctions and desmosomes

12.5 Integrins mediate cell-ECM adhesions (hemidesmosomes)

12.6 Tight junctions and Gap junctions

12.7 The Basal Lamina and Laminin in the Basal Lamina

12.8 Type IV collagen and Perlecan in the Basal Lamina

12.9 The Extracellular Matrix II: P Connective Tissue

12.10 Key ECM Components and Fibrillar Collagens in Connective tissue

12.11 Proteoglycans, Fibronectins, and Elastic Fibers in Connective tissue 

13.Stem cells, cell asymmetry and cell death

13.1 Cell lineage and Asymmetric cell division

13.2 Fertilization, Cleavage and Inner cell mass

13.3 ES cells, Animal Cloning and iPs cells

13.4 Patterns of Stem-cell Differentiation and Germ-line stem cells

13.5 Tissue Stem Cells

13.6 Common Hierarchy of Cell Polarization and Asymmetry

13.7 Mechanisms of cell polarity and asymmetric cell division

13.8 Apoptosis and necrosis

13.9 Evolutionarily Conserved Proteins Participate in the Apoptotic Pathway

13.10 Mitochondria Play a Central Role in Regulation of Apoptosis

13.11 Death Signals Promote Cell Murder by Activating Caspases

14.Cancer

14.1 Cancer—malignant tumors

14.2 Cell origin and environment required for its development

14.3 Specific mutations transform cultured cells into tumor cells

14.4 Gain-of-function mutations convert proto-oncogenes into oncogenes

14.5 Loss-of-function mutations in tumor-suppressor genes are oncogenic

14.6 Cancer Misregulation of Growth Regulatory Pathways

14.7 Cancer and Mutation of cell division and checkpoint regulators

14.8 Carcinogens and caretaker genes in cancer 

                              Final exam

                           



参考资料

1.         Molecular Cell Biology, 7th Edition, Harvey Lodish/Arnold Berk, etc., 2013,  Macmillan                   Higher Education

2.           Molecular Biology of The Cell, 6th Edition, Alberts B., et al., John Wilson/Tim  Hunt, 2015,                Garland Science