课程详情
课程评价
spContent=细胞生物学是从事生物医学研究所必须掌握的课程之一。本课程为中英文双语教学,目的是让同学们尽快熟悉细胞生物学领域内的英文词汇,为掌握最新进展和提高自学能力奠定基础。欢迎对生命科学感兴趣,或者准备进一步深造,报考研究生,立志将来从事生命科学研究的同学选修本课程。
—— 课程团队
课程概述

细胞生物学是生命科学重要的基础学科之一,是分子生物学与个体生物学之间承上启下的学科。本课程主要是让学生了解细胞的基本结构及其功能,熟悉细胞生命活动的基本特点及其分子机制,并了解当前细胞生物学的发展动态和国际前沿知识。本课程为中英文双语教学,即授课用中文,而PPT,教材及考试都为英文。适合对生物感兴趣,或者立志将来从事生命科学研究的学生进行学习。


授课目标

我们期待通过本课程的双语教学,使同学们能熟练掌握细胞生物学的基本概念和基本原理。熟悉相关领域内的关键词汇,提高同学们的阅读英文文献的能力,使同学们获得运用所学知识去分析科技文献中数据,跟踪本学科领域内的最新进展的能力。

课程大纲

1.Introduction of Molecular Cell Biology

1.1 Research objectives of the cell biology

1.2 Microscope and Cell theory

1.3 Major events in cell biology

2.Culturing,visualizing cells

2.1 Growing Cells in Culture
2.2 Monoclonal antibodies

2.3 Fluorescence Microscopy

2.4 Flow cytometry  

2.5 Spinning Disk Confocal Microscopy

2.6 FRAP and FRET

2.7 RNA interference

3.Biomembrane structure and Transmembrane transport of ions  and small molecules

3.1 The structrure of plasmic membrane

3.2 The lipid bilayer:Composition and structural organization

3.3 Forms of lipid molecules exist in water

3.4 Lipids Movement in biomembrane

3.5 Structure of Membrane Proteins

3.6 Synthesis of Phospholipids and Intracellular Movement

3.7 Overview of Transmembrane transport

3.8.Facilitated Transport of Glucose and Water

3.9 ATP –powered pumps

3.10 Na+/k+ pump

3.11 Ca2+ pump

3.12 K+ channel and the Resting membrane potencial

3.13 Cotransport by Symporters and Antiporters

3.14 Transcellular transport

4. Cellular Energetics

4.1 Overview of aerobic oxidation

4.2 Structure of Mitochondria

4.3 Mitochondrial Oxidation of Fatty Acids Generates ATP

4.4 The Electron Carriers and Electron Transport Chain

4.5 The Electron Transport and Generation of the Proton-Motive Force

4.6 Chemiosmotic theory  and Endosymbiont hypothesis  

4.7 Harnessing the Proton-Motive Force to synthesize ATP

5. Moving proteins into membrane and organells

5.1 Targeting Proteins to and Across the ER Membrane

5.2 Insertion of single-pass Proteins into the ER

5.3 Insertion of Multipass Proteins into the ER

5.4 Protein Glycosylation and Folding in the ER

5.5 Formation of disulfide bonds in the ER

5.6 Protein Quality Control in the ER

5.7Targeting of Proteins to Mitochondria

5.8 Targeting of Peroxisomal Proteins

6.Vesicular traffic,secretion and endocytosis

6.1 Molecular Mechanisms of Vesicle Budding and Fusion

6.2 Early Stages of the Secretory Pathway

6.3 Later Stages of the Secretory Pathway

6.4 Receptor-Mediated Endocytosis

6.5The structure and functions of Lysosomes

6.6 Directing Membrane Proteins and Cytosolic Materials to the Lysosome

7.Signal transduction

7.1 Brief Introduction of Cell Communication

7.2 Signaling Molecules and Receptors

7.3 G Proteins and G Protein-coupled Receptors (GPCR)

7.4 GPCR Mediated Signaling pathways

8.Signaling pathways that control  gene activity

8.1 Protein Tyrosine Kinases (RTKs) and RTK-Ras Signaling Pathway

8.2 Phosphoinositide Signaling Pathways

8.3 TGF-b Receptors and TGFb Signaling Pathways

8.4 Cytokine receptors and JAK-STAT Signaling Pathways

8.5 Signaling Pathways controlled by Ubiquitination

8.6 Integration of Multiple Signaling Pathways

9: I.Microfilaments: Actin Structures

9.1 Actin Filaments

9.2 Dynamic Assembly of Actin Filaments

9.3 Actin-Based Cellular Structures

9.4 Myosins and Myosin-Powered Movements

9.5 Mechanisms of Cell Migration

10. II Microfilaments: Microtubules and Intermediate Filament

10.1  Structure and Organization of Microtubule

10.2  Regulation of Microtubule Dynamics

10.3  Microtubule-Based Motor Proteins

10.4  Intermediate Filaments

10.5  Coordination of Cellular Cytoskeleton

11.The Eukaryotic cell cycle

11.1. Overview of the cell cycle and its control

11.2. Model organisms and methods to study the cell cycle

11.3. CDK and Its Discovery

11.4. Regulation of CDK Activity

11.5. Commitment to the Cell Cycle and DNA Replication

11.6. Entry into Mitosis and Completion of Mitosi

11.7. Surveillance mechanisms in cell cycle regulation

11.8 Meiosis: A Special Type of Cell Division

12.Cell senescence and death I

12.1 Introduction of cell senescence

12.2 Theories and mechanisms of cell senescence

12.3 Physiological and pathological roles of cell senescence

      Cell senescence and death II

12.4 Introduction of cell death

12.5 Mechanism of cell death

12.6 Physiological and pathological roles of cell death

13.Integrating cells into tissues

13.1 An Overview on Cell-Cell and Cell-Matrix Adhesion

13.2 Type of Cell Junctions and Anchoring Junction

13.3 Tight junctions and Gap junctions

13.4 ECMs of The Basal Lamina

13.5 ECMs of Connective tissu

14. Stem cells, cell asymmetry and cell death

14.1 Early metazoan developmnt and embryonic stem cells

14.2 Stem Cells and Niches in Multicellular Organism

14.3 Mechanisms of cell polarity and asymmetric cell division


预备知识

本课程的授课对象为大学二年级学生,前期课程应已修完生物化学、有机化学等基础学科。


证书要求


本课程的学习过程包含:观看讲课视频、完成课后作业及讨论,参加期中,期末考试。

课程学习的最终成绩由一下几个部分组成:

(1) 单元测验成绩占40%

(2)期中考试成绩占30%

(3) 期末考试成绩占30%

在4月20日进行期中考试,6月7 日进行期末考试,期中考试前的内容不再考察。

完成课程学习并考核合格(>=60)的可获得合格证书,成绩优秀(>80)的可获得优秀证书。

 




参考资料

1.         Molecular Cell Biology, 7th Edition, Harvey Lodish/Arnold Berk, etc., 2013,  Macmillan                   Higher Education

2.           Molecular Biology of The Cell, 6th Edition, Alberts B., et al., John Wilson/Tim  Hunt, 2015,                Garland Science