课程详情
课程评价
spContent=细胞生物学是从事生物医学研究所必须掌握的课程之一。本课程为中英文双语教学,目的是让同学们尽快熟悉细胞生物领域内的英文词汇,为掌握最新进展和提高自学能力奠定基础。欢迎对生命科学感兴趣,或者准备进一步深造,报考研究生,立志将来从事生命科学研究的同学选修本课程。
—— 课程团队
课程概述

细胞生物学是生命科学重要的基础学科之一,是分子生物学与个体生物学之间承上启下的学科。本课程主要是让学生了解细胞的基本结构及其功能,熟悉细胞生命活动的基本特点及其分子机制,并了解当前细胞生物学的发展动态和国际前沿知识。本课程为中英文双语教学,即授课用中文,而PPT,教材及考试都为英文。适合对生物感兴趣,或者立志将来从事生命科学研究的学生进行学习。


授课目标

我们期待通过本课程的双语教学,使同学们能熟练掌握细胞生物学的基本概念和基本原理。熟悉相关领域内的关键词汇,提高同学们的阅读英文文献的能力,使同学们获得运用所学知识去分析科技文献中数据,跟踪本学科领域内的最新进展的能力。

课程大纲

Introduction of Molecular cell biology

The objectives and development of cell biology

Microscope and Cell theory

Major events in cell biology

Cell theory-test

Culturing and visualizing cells

Culturing and visualizing cells-test

Growing Cells in Culture

Monoclonal antibodies

Fluorescence Microscopy

Flow cytometry

Spinning Disk Confocal Microscopy

FRAP and FRET

RNA interference

Biomembrane Structure

The structrures of the plasmic membrane

The lipid bilayer:  Composition and  structural organization

Forms of lipid molecules exist in water

Lipids Movement in biomembrane

Structure of Membrane Proteins

Synthesis of Phospholipids and Intracellular Movement

Biomembrane Structure-test

Transmembrane transport of Ions and small molecules

Overview of transmembrane transport

Facilitated Transport of Glucose and Water

ATP –powered pumps

Na+/k+ pump

Ca2+ pump

K+ channel and the Resting membrane potencial

Cotransport by Symporters and Antiporters

Transcellular transport

Transmembrane transport of Ions and small molecules

Cellular Energetics

Electron Transport and Reactive Oxygen Species (ROS)

Overview of aerobic oxidation

Structure of Mitochondria

Mitochondrial Oxidation of Fatty Acids Generates ATP

The Electron Carriers and Electron Transport Chain

The Electron Transport and Generation of the Proton-Motive Force

Chemiosmotic theory  and Endosymbiont hypothesis

Harnessing the Proton-Motive Force to synthesize ATP

Cellular Energetics

Moving Proteins into Membranes and Organelles

Moving Proteins into Membranes and Organelles

Targeting Proteins to and Across the ER Membrane

Insertion of single-pass Proteins into the ER

Insertion of Multipass Proteins into the ER

Protein Glycosylation and  Folding in the ER

Formation of disulfide bonds in the ER

Protein Quality Control in the ER

Targeting of Proteins to Mitochondria

Targeting of Peroxisomal Proteins

Vesicular   traffic,secretion and endocytosis

Molecular Mechanisms of Vesicle Budding and Fusion

Early Stages of the Secretory Pathway

Later Stages of the Secretory Pathway

Receptor-Mediated Endocytosis

Directing Membrane Proteins and Cytosolic Materials to the Lysosome

The structure and functions of Lysosomes

Vesicular traffic,secretion and endocytosis

Signal transduction  and G protein-coupled receptors

Brief Introduction of Cell Communication

Signaling Molecules and Receptors

G Proteins and G Protein-coupled Receptors (GPCR)

GPCR Mediated Signaling pathways

Signal transduction

Signaling pathways that control  gene activity

Outlines of Signaling pathways that control  gene activity

Phosphoinositide Signaling Pathways

TGF-b Receptors and TGFb Signaling Pathways

Signaling Pathways controlled by Ubiquitination

The Ras/MAPK and JAK/STAT  signaling pathway

Signaling pathways that control gene activity

Cell   organization and movement I: Microfilaments

Actin Filaments

Dynamic Assembly of Actin Filaments

Actin-Based Cellular Structures

Myosins and Myosin-Powered Movements

Cell organization and movement I: Microfilament

Cell   organization and movement II: Microtubules and intermediate filament

第一单元 Microtubule Structure and Organization

第二单元 Microtubule Dynamics

第三单元 Regulation of Microtubule Structure and Dynamics

第四单元 Kinesins & Dyneins: Microtubule-Based Motor Proteins

第五单元 Cilia & Flagella: Microtubule-Based  Surface Structures

第六单元 Mitotic Spindle

第七单元 Intermediate Filaments

第八单元 Coordination and Cooperation Between Cytoskeletal Elements

Cell organization and movement II: Microtubules and intermediate filamen

Nucleus, chromatin and chromosomes

第一单元 Unit 1, Nuclear Envelope (NE), Nuclear Pore Complex (NPC) & Nuclear Transport

第二单元 Unit 2, Structural Organization of Eukaryotic Chromosomes

第三单元 Unit 3, Morphology and Functional Elements of Eukaryotic Chromosomes

第四单元 Unit 4, Nucleolus

Nucleus, chromatin and chromosomes 单元测验

The Eukaryotic Cell Cycle

The Eukaryotic Cell Cycle-test

第一单元 Unit 1, Overview of the Cell Cycle and Its Control

第二单元 Unit 2, Model Organisms and Methods to Study the Cell Cycle

第三单元 Unit 3, CDK and Its Discovery

第四单元 Unit 4, Regulation of CDK Activity

第五单元 Unit 5, Commitment to the Cell Cycle and DNA Replication

第六单元 Unit 6, Entry into Mitosis and Completion of Mitosis

第七单元 Unit 7, Surveillance Mechanisms in Cell Cycle Regulation

第八单元 Unit 8, Meiosis: A Special Type of Cell Division

Integrating cells into tissues

Ch20 Test -single answer choice

An Overview on Cell-Cell and Cell-Matrix Adhesion

Type of Cell Junctions and Anchoring Junctions

Tight junctions and Gap junctions

ECMs of The Basal Lamina

ECMs of Connective tissue

Stem cells, cell asymmetry and cell death

Early metazoan developmnt and embryonic stem cells

Stem Cells and Niches in Multicellular Organisms

Mechanisms of cell polarity and asymmetric cell division

Cell death and its regulation

Ch21 Test- single answer Choice

预备知识

本课程的授课对象为大学二年级学生,前期课程应已修完生物化学、有机化学等基础学科。


证书要求


本课程的学习过程包含:观看讲课视频、完成课后作业及讨论,参加期中,期末考试。

课程学习的最终成绩由一下几个部分组成:

(1) 单元测验成绩占40%

(2)期中考试成绩占30%

(3) 期末考试成绩占30%

在11月11日进行期中考试,12月23日进行期末考试,期中考试前的内容不再考察。

完成课程学习并考核合格(>=60)的可获得合格证书,成绩优秀(>80)的可获得优秀证书。

 




参考资料

1.         Molecular Cell Biology, 7th Edition, Harvey Lodish/Arnold Berk, etc., 2013,  Macmillan                   Higher Education

2.           Molecular Biology of The Cell, 6th Edition, Alberts B., et al., John Wilson/Tim  Hunt, 2015,                Garland Science